On Saturday around 5:30 p.m., Dryden OPP officers responded to a multi-vehicle collision on Highway 17 in Machin Township, west of Vermilion Bay.
An eastbound SUV lost control and collided with a westbound tractor-trailer unit.
The Dryden & Kenora Ambulance and Machin Fire Department attended the scene to assist the occupants of the vehicles.
The driver and front passenger of the SUV died as a result of the collision while the other three passengers in the back were transported to hospital for treatment.
They later were airlifted by ORNGE air ambulance to Winnipeg for further treatment.
The deceased have been identified as Dustin McLaughlin, 31, of Portage La Prairie, Man. and Cheyanne McLaughlin, 13, of Nipigon, Ont.
The lone occupant of the tractor-trailer unit was not hurt.
The Technical Traffic Collision Investigation unit attended the scene to conduct its investigation.
Highway 17 was closed for about nine hours.