Dryden OPP officers responded to a two-vehicle collision around 1:10 a.m. yesterday on Highway 17 at Airport Road, just east of Dryden.
Police confirmed the three occupants of one of the vehicles were killed.
The two occupants of the other vehicle sustained non-life threatening injuries.
The victims were identified as Aaron Thrones, 20, Todd Bousfield, 23, and Davin Arnold, 23, all of Dryden.
Highway 17 was closed for about six hours to remove the vehicles but now is fully open to traffic.
The investigation is continuing but charges are not pending.